Rat/Mouse Cytochrome c Quantikine ELISA Kit

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
2.5 hours
Sample Type & Volume Required Per Well
Cell Lysates (50 uL), Subcellular Fractions (50 uL)
Sensitivity
0.5 ng/mL
Assay Range
0.8 – 25 ng/mL (Cell Lysates, Subcellular Fractions)
Specificity
This kit recognizes both the reduced and oxidized forms of natural rat and mouse cytochrome c.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested. Interference No significant interference observed with available related molecules. Product Summary The Quantikine Rat/Mouse Cytochrome c Immunoassay is a 2.5 hour solid phase ELISA designed to measure rat and mouse cytochrome c. It contains natural rat cytochrome c and antibodies raised against cytochrome c, and because of the 100% sequence identity of rat and mouse cytochrome c, this kit can be used to accurately quantitate natural rat or mouse cytochrome c in cell lysates and subcellular fractions. Background: Cytochrome c Cytochrome c is a small, highly conserved, heme-containing protein involved in mitochondrial electron transport. Cytochrome c is released from the mitochondria in response to pro-apoptotic stimuli and serves as a cofactor in initiating the activation of the APAF-1/Caspase-9 complex. The effect of specific proteins and/or small molecules on the initiation of the intrinsic apoptotic pathway can be evaluated in an in vitro system of isolated mitochondria by monitoring the release of cytochrome c. Entrez Gene IDs: 54205 (Human) Alternate Names: CYCHCS; CYCS; Cytochrome c; cytochrome c, somatic; THC4

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